Blood Doping Identification
One of the central projects for the lab, which has garnered research support from the World Anti-Doping Agency and the US Anti-Doping Agency, has been the development of CE methods to identify autologous blood transfusions. The lab is developing means of identifying the physical and biochemical changes that occur in red blood cells while in storage. By accentuating these differences we are able to differentiate the stored and fresh cells once mixed via a prohibited, autologous blood transfusion. This will become a key component in the continuing fight against doping in sports.
In our ongoing development of CE methods for the separation of biologically relevant compounds the group has been investigating how self-assembled surface coatings can be incorporated and modified in CE separations. The aim of the lab is to develop better options for bioseparations, and to devise new means of altering, and controlling electrophoretic separations.
The ability to sequester microliter into nanoliter volumes of sample in aqueous droplets surrounded by perflorinated carbon liquids has proved to be highly useful for chemical and biochemical analyses. Our work is focused on both the controlled formation and manipulation of these aqueous droplets and the ability to seamlessly integrate this system into a separation system. We are focusing on the development of a system that will allow the delivery of aqueous droplets to a capillary electrophoresis system, allowing for the rapid and extensive analysis of the droplet contents.
Several projects are currently underway in the lab making use of CE’s unique capability to separate molecular complexes, and in the process, probe aspects about the complex formations and equilibria. We are applying these capabilities in collaboration with groups in biochemistry, biology, and industry to better understand the complex systems they are studying.
Chris Harrison began his study of chemistry at Concordia University in Montreal, following a Ph.D. at the University of Alberta with Chuck Lucy (2005) he proceed to a postdoc with Michael Bowser at the University of Minnesota Twin Cities. These opportunities exposed Chris to a wide breadth of analytical separation techniques, from HPLC, to microfluidic systems.
In 2007 Chris joined the faculty at SDSU where he has established his research group, and become recognized by his peers as being at the forefront of advancing the use of technology in teaching analytical chemistry.
When not in the lab Chris tries to spend as much time as possible enjoying the wonderful San Diego weather by spending hours riding his bike throughout the county.
Mike Appel is a San Diego native and chemistry graduate student at SDSU. After receiving his Bachelor’s degree in the entomological sciences at UC Riverside he joined Dr. Harrison’s lab with an interest in widening his understanding of chemical analysis to help him work toward a future career within the field of forensics. Currently Mike is conducting research using capillary electrophoresis to observe phage interactions with mucin.
Outside of his education, Mike enjoys working as a martial arts and self-defense instructor of kids and adults and hiking when he has free time.
Jessica Torres is a chemistry major and has worked in the Harrison group for the past four semesters. She is currently investigating the use of polyhexamethylene biguanide (PHMB) as a capillary coating and its potential use in separating fresh red blood cells from aged ones in the Blood Doping Identification project. In her spare time she enjoys playing Animal Crossing, dismantling the patriarchy, and hanging out with her dogs.
Amy Vo did her undergraduate studies at UCSD in biochemistry and cellular biology with a minor in environmental systems. Following graduation, she worked as a QC/QA analyst in several industries including food and cosmetic. However, it was at Phenomenex (Torrrance, CA) where she discovered that she really like to work with technical instruments such as HPLC and GC to take on analytical problems in bioanalysis.
Since becoming a graduate student at SDSU, Amy has been working on two projects. Her main project is to design, build and characterize the Coaxial CE Droplet System (CCEDS). The aim of this project is to create a device capable of electrophoretic separation and encapsulation of separated components in monodispersed droplets post column analysis. With her second project, Amy is using CE to investigate the strength of molecular interaction between phage and mucin.
When she is not in the lab, she likes to travel and hunt for fruit around the world (a fruit hunter, it’s a thing, she said). She also a political junkie and a lover of great fictions. She is set to graduate in May 2017 and vows to continue to be curious and to slay dragons wherever they appear.
Sangho (Davie) Yun is a graduate student at SDSU pursuing masters in Analytical Chemistry. After receiving his bachelor’s degree in
chemistry from University of California, Los Angeles, he worked at
Johnson & Johnson managing formulation/pre-formulation development for
all in-house animal toxicological studies. He is currently working on
autologous blood doping project, in attempt to find an optimal method
to detect what is considered illegal by the World Anti-Doping Agency.
Outside of school, Davie enjoys watching movies, playing games, and
hanging out with his friends over cup of coffee (and beer).
Michael Dalman is proud to pursue his undergraduate chemistry degree in his hometown of San Diego. Michael is evidently a masochist since he hopes to become a high school chemistry teacher upon graduation. He first researched chemistry at SDSU under Dr. Cooksy as a high school senior. Since attending San Diego State, his interests in physical and analytical chemistry have continued to grow.
Outside of school, Michael enjoys skydiving, backpacking, and sleeping.
Samantha Daoud is an undergraduate student at San Diego State University. She is pursuing a Bachelors degree in Biochemistry and is new to research. Samantha will be working on the steroid analysis project and will be graduating in May. She volunteers at the San Diego Center for the Blind and is working on pursuing a career in Optometry.
When Samantha is not in school or working she likes to use her spare time to hang out with friends and family. Her hobbies also include photography/videography and singing.
Keifer Garcia is an undergrad who is a 4th year. I hope to work on the blood doping project as that interests me the most and for now, and I hope to get into it in the future using all the knowledge I obtained from shadowing different students. My interests in Chemistry usually relate to the Biochemistry aspect, however, I am still open to other areas in the field. I am thinking of working on protein synthesis with my degree in the future.
Ashley Huskey– Ashley is an undergraduate at San Diego State University pursuing a degree in Chemistry. After graduation, she plans on attending pharmacy school. Ashley is a new member of the lab and is investigation the electrophoretic mobility of hemoglobin complexes. In her spare time Ashley enjoys camping, snowboarding and cooking.
Sheridan Kazek– Sheridan Kazek is a new member to the Harrison lab. She is an undergraduate student at SDSU pursuing a Bachelors degree in Chemistry and aiming to continue her studies to become a dental hygienist after graduation. Sheridan is working on the steroid analysis project, where she is trying to develop a capillary electrophoretic separation of the derivatized compounds in a new approach to anti-doping analysis. In her free time, Sheridan enjoys rock climbing, hiking, and basically anything outdoors.
Madee Noroña – Madison (Madee) Noroña is new to the lab as an undergraduate student majoring in chemistry. She enjoys hip hop dance and repping the Bay Area wherever she goes.
Kai Nguyen – Kai is currently a junior majoring in Chemistry. He began researching in the lab in Fall 2017 under the guidance of Sangho Yun, he is currently working on the direct fluorination of fused silica capillaries with hopes to help improve protein separations. While not on campus, Kai is currently interning at Alan Laboratory. When not in the lab, Kai enjoys playing badminton and playing video games.
Jaclyn Pittman is an undergraduate student at SDSU studying Environmental Science and Chemistry. She plans to enroll in a doctoral program upon graduating and pursue a career in environmental chemistry. She is currently working on the MPXA complexation project, finding it both very intriguing and challenging.
When she is not studying or working in the lab, Jaclyn enjoys going to the beach, attending concerts, and volunteering with the children’s ministry at her church.
Phillip Tang is a third year majoring in Chemistry and began research in the fall of 2017, working with Jaclyn on the MPXA complexation project. Ideally, Phillip would be pursuing pharmacy school after graduation.
Outside of school work and lab, Phillip enjoys immersing himself in video games, watching videos of people playing those games, and playing with cats. You can typically find Phillip in the corner of the lab hunched over his phone, headphones on, watching videos or doing school work.
Wells, S. S., De La Toba, E., Harrison, C. R. (2016). Metal cation control of electroosmotic flow magnitude in phospholipid coated capillaries. ELECTROPHORESIS, 37(10), 1303-1309.
Harrison, C. R., Fang, J. C.-Y., Walthall, K. J., Green, C. C., Porobic, V. (2014). Towards the identification of autologous blood transfusions through capillary electrophoresis. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 406(3), 679-686.
Morris, A. L., Harrison, C. R. (2013). Adsorption of buffer ion pairs can alter long-term electroosmotic flow stability. ELECTROPHORESIS, 34(17), 2585-2592.
Harrison, C. R. (2013). Role of Capillary Electrophoresis in the Fight Against Doping in Sports. ANALYTICAL CHEMISTRY, 85(15), 6982-6987.
Harrison, C. R., Vydha, S. (2012). Capillary electrophoretic analysis of whole blood samples for hemoglobin-based oxygen carriers without the use of immunoprecipitation. ELECTROPHORESIS, 33(7), 1087-1094.
Harrison, C. R., Sader, J. A., Lucy, C. a. (2006). Sulfonium and phosphonium, new ion-pairing agents with unique selectivity towards polarizable anions. JOURNAL OF CHROMATOGRAPHY A, 1113(1-2), 123-129.
Harrison, C. R., Lucy, C. A. (2002). Determination of zwitterionic and cationic surfactants by high-performance liquid chromatography with chemiluminescenscent nitrogen detection. JOURNAL OF CHROMATOGRAPHY A, 956(1-2), 237-244.
Lucy, C. A., Harrison, C. (2001). Chemiluminescence nitrogen detection in ion chromatography for the determination of nitrogen-containing anions. JOURNAL OF CHROMATOGRAPHY A, 920(1-2), 135-141.